@article {das_single_2021,
	title = {Single toxicity of arsenic and combined trace metal exposure to a microalga of ecological and commercial interest: Diacronema lutheri},
	journal = {Chemosphere},
	year = {2021},
	pages = {132949},
	abstract = {Eco-toxicological assays with species of economic interest such as Diacronema lutheri are essential for industries that produce aquaculture feed, natural food additives and also in drug developing industries. Our study involved the exposure of a single and combined toxicity of arsenic (As V) to D. lutheri for the entire algal growth phase and highlighted that a combined exposure of As V with other essential (Copper, Cu; Nickel, Ni) and non-essential (Cadmium, Cd; Lead, Pb) trace metals reduced significantly the cell number, chlorophyll a content, and also significantly increased the de-epoxidation ratio (DR) as a stress response when compared to the single toxicity of As V. Arsenic, as one of the ubiquitous trace metal and an active industrial effluent is reported to have an increased bio-concentration factor when in mixture with other trace metals in this study. In the combined exposure, the concentration of total As bio-accumulated by D. lutheri was higher than in the single exposure. Hence, polluted areas with the prevalence of multiple contaminants along with the highly toxic trace metals like As can impose a greater risk to the exposed organisms that may get further bio-magnified in the food chain. Our study highlights the consequences and the response of D. lutheri in terms of contamination from single and multiple trace metals in order to obtain a safer biomass production for the growing need of natural derivatives.},
	keywords = {Arsenic, bioaccumulation, Bioconcentration factor, Chlorophyll, de-epoxidation ratio, RCC1537, Trace metals mixture},
	issn = {0045-6535},
	doi = {10.1016/j.chemosphere.2021.132949},
	url = {https://www.sciencedirect.com/science/article/pii/S0045653521034214},
	author = {Das, Shagnika and Gevaert, Fran{\c c}ois and Ouddane, Baghdad and Duong, Gwendoline and Souissi, Sami}
}
