@article {castejon_limpet_2022,
	title = {Limpet larvae (Patella aspera R{\"o}ding, 1798), obtained by gonad dissection and fecundation in vitro, settled and metamorphosed on crustose coralline algae},
	journal = {Journal of the Marine Biological Association of the United Kingdom},
	year = {2022},
	note = {Publisher: Cambridge University Press},
	month = {feb},
	pages = {1{\textendash}12},
	abstract = {<p>The limpet Patella aspera R{\"o}ding, 1798, is a native species from the Macaronesian region whose fishing is regulated. The early life of limpets, including the settlement process, is poorly known thus far. The current study evaluated different substrates to induce settlement in P. aspera, including microalgae strains (Halamphora coffeaeformis, Navicula incerta and Pavlova sp.) and crustose coralline algae (CCA) obtained from limpet shells. The results showed that gametes obtained by dissection and matured artificially using alkalinized seawater baths can produce viable larvae able to metamorphose to juveniles. Feeding was not required during larval development, suggesting lecithotrophy. Early postlarvae were identified by the shedding of the velum, and juveniles were identified by teleoconch and active grazing behaviour. The presence of CCA shortened the timing for settlement and increased the ratio of juveniles. The type and abundance of CCA can influence settlement success. Moreover, the results suggested that settlement and metamorphosis in true limpets (Patellogastropoda) might be triggered by a two-step mechanism, i.e. a first cue influencing the shift between swimming and crawling activity and a second cue determining settlement and metamorphosis to early postlarvae and juveniles.</p>
},
	keywords = {Early postlarvae, lecithotrophy, metamorphosis, oocyte alkalinization, pediveliger larvae, RCC3458, settlement},
	issn = {0025-3154, 1469-7769},
	doi = {10.1017/S0025315421000916},
	author = {Castej{\'o}n, Diego and Nogueira, Natacha and Andrade, Carlos A. P.}
}
