%0 Journal Article %J Elementa: Science of the Anthropocene %D 2020 %T Culturable diversity of Arctic phytoplankton during pack ice melting %A Ribeiro, Catherine Gérikas %A dos Santos, Adriana Lopes %A Gourvil, Priscillia %A Le Gall, Florence %A Marie, Dominique %A Tragin, Margot %A Probert, Ian %A Vaulot, Daniel %K RCC5197 %K RCC5198 %K RCC5199 %K RCC5200 %K RCC5201 %K RCC5202 %K RCC5203 %K RCC5204 %K RCC5205 %K RCC5206 %K RCC5207 %K RCC5208 %K RCC5209 %K RCC5210 %K RCC5211 %K RCC5212 %K RCC5213 %K RCC5214 %K RCC5215 %K RCC5216 %K RCC5217 %K RCC5218 %K RCC5219 %K RCC5220 %K RCC5221 %K RCC5222 %K RCC5223 %K RCC5224 %K RCC5225 %K RCC5226 %K RCC5227 %K RCC5228 %K RCC5229 %K RCC5230 %K RCC5231 %K RCC5232 %K RCC5233 %K RCC5234 %K RCC5235 %K RCC5236 %K RCC5237 %K RCC5238 %K RCC5239 %K RCC5240 %K RCC5241 %K RCC5242 %K RCC5243 %K RCC5244 %K RCC5245 %K RCC5246 %K RCC5247 %K RCC5248 %K RCC5249 %K RCC5250 %K RCC5251 %K RCC5252 %K RCC5253 %K RCC5254 %K RCC5255 %K RCC5256 %K RCC5257 %K RCC5258 %K RCC5259 %K RCC5260 %K RCC5261 %K RCC5262 %K RCC5263 %K RCC5264 %K RCC5265 %K RCC5266 %K RCC5267 %K RCC5268 %K RCC5269 %K RCC5270 %K RCC5271 %K RCC5272 %K RCC5273 %K RCC5274 %K RCC5275 %K RCC5276 %K RCC5277 %K RCC5278 %K RCC5279 %K RCC5280 %K RCC5281 %K RCC5282 %K RCC5283 %K RCC5284 %K RCC5285 %K RCC5286 %K RCC5287 %K RCC5288 %K RCC5289 %K RCC5290 %K RCC5291 %K RCC5292 %K RCC5293 %K RCC5294 %K RCC5295 %K RCC5296 %K RCC5297 %K RCC5298 %K RCC5299 %K RCC5300 %K RCC5301 %K RCC5302 %K RCC5303 %K RCC5304 %K RCC5305 %K RCC5306 %K RCC5307 %K RCC5308 %K RCC5309 %K RCC5310 %K RCC5311 %K RCC5312 %K RCC5313 %K RCC5314 %K RCC5315 %K RCC5316 %K RCC5317 %K RCC5318 %K RCC5319 %K RCC5320 %K RCC5321 %K RCC5322 %K RCC5323 %K RCC5324 %K RCC5325 %K RCC5326 %K RCC5327 %K RCC5328 %K RCC5329 %K RCC5330 %K RCC5331 %K RCC5332 %K RCC5333 %K RCC5334 %K RCC5335 %K RCC5336 %K RCC5337 %K RCC5338 %K RCC5339 %K RCC5340 %K RCC5341 %K RCC5342 %K RCC5343 %K RCC5344 %K RCC5345 %K RCC5346 %K RCC5347 %K RCC5348 %K RCC5349 %K RCC5350 %K RCC5351 %K RCC5352 %K RCC5353 %K RCC5354 %K RCC5355 %K RCC5356 %K RCC5357 %K RCC5358 %K RCC5359 %K RCC5360 %K RCC5361 %K RCC5362 %K RCC5363 %K RCC5364 %K RCC5365 %K RCC5366 %K RCC5367 %K RCC5368 %K RCC5369 %K RCC5370 %K RCC5371 %K RCC5372 %K RCC5373 %K RCC5374 %K RCC5375 %K RCC5376 %K RCC5377 %K RCC5378 %K RCC5379 %K RCC5380 %K RCC5381 %K RCC5382 %K RCC5383 %K RCC5384 %K RCC5385 %K RCC5386 %K RCC5387 %K RCC5388 %K RCC5389 %K RCC5390 %K RCC5391 %K RCC5392 %K RCC5393 %K RCC5394 %K RCC5395 %K RCC5396 %K RCC5397 %K RCC5398 %K RCC5399 %K RCC5400 %K RCC5401 %K RCC5402 %K RCC5403 %K RCC5404 %K RCC5405 %K RCC5406 %K RCC5407 %K RCC5408 %K RCC5409 %K RCC5410 %K RCC5411 %K RCC5412 %K RCC5413 %K RCC5414 %K RCC5415 %K RCC5416 %K RCC5417 %K RCC5418 %K RCC5419 %K RCC5420 %K RCC5421 %K RCC5422 %K RCC5423 %K RCC5424 %K RCC5425 %K RCC5426 %K RCC5427 %K RCC5428 %K RCC5429 %K RCC5430 %K RCC5431 %K RCC5432 %K RCC5433 %K RCC5434 %K RCC5435 %K RCC5436 %K RCC5437 %K RCC5438 %K RCC5439 %K RCC5440 %K RCC5441 %K RCC5442 %K RCC5443 %K RCC5444 %K RCC5445 %K RCC5446 %K RCC5447 %K RCC5448 %K RCC5449 %K RCC5450 %K RCC5451 %K RCC5452 %K RCC5453 %K RCC5454 %K RCC5455 %K RCC5456 %K RCC5457 %K RCC5458 %K RCC5459 %K RCC5460 %K RCC5461 %K RCC5462 %K RCC5463 %K RCC5464 %K RCC5465 %K RCC5466 %K RCC5467 %K RCC5468 %K RCC5469 %K RCC5470 %K RCC5471 %K RCC5472 %K RCC5473 %K RCC5474 %K RCC5475 %K RCC5476 %K RCC5477 %K RCC5478 %K RCC5479 %K RCC5480 %K RCC5481 %K RCC5482 %K RCC5483 %K RCC5484 %K RCC5485 %K RCC5486 %K RCC5487 %K RCC5488 %K RCC5489 %K RCC5490 %K RCC5491 %K RCC5492 %K RCC5493 %K RCC5494 %K RCC5495 %K RCC5496 %K RCC5497 %K RCC5498 %K RCC5499 %K RCC5500 %K RCC5501 %K RCC5502 %K RCC5503 %K RCC5504 %K RCC5505 %K RCC5506 %K RCC5507 %K RCC5508 %K RCC5509 %K RCC5510 %K RCC5511 %K RCC5512 %K RCC5513 %K RCC5514 %K RCC5515 %K RCC5516 %K RCC5517 %K RCC5518 %K RCC5519 %K RCC5520 %K RCC5521 %K RCC5522 %K RCC5523 %K RCC5524 %K RCC5525 %K RCC5526 %K RCC5527 %K RCC5528 %K RCC5529 %K RCC5530 %K RCC5531 %K RCC5532 %K RCC5533 %K RCC5534 %K RCC5535 %K RCC5536 %K RCC5537 %K RCC5538 %K RCC5539 %K RCC5540 %K RCC5541 %K RCC5542 %K RCC5543 %K RCC5544 %K RCC5545 %K RCC5546 %K RCC5547 %K RCC5548 %K RCC5549 %K RCC5550 %K RCC5551 %K RCC5552 %K RCC5553 %K RCC5554 %K RCC5555 %K RCC5556 %K RCC5557 %K RCC5558 %K RCC5559 %K RCC5560 %K RCC5561 %K RCC5562 %K RCC5563 %K RCC5564 %K RCC5565 %K RCC5566 %K RCC5567 %K RCC5568 %K RCC5569 %K RCC5570 %K RCC5571 %K RCC5572 %K RCC5573 %K RCC5574 %K RCC5575 %K RCC5576 %K RCC5577 %K RCC5578 %K RCC5579 %K RCC5580 %K RCC5581 %K RCC5582 %K RCC5583 %K RCC5584 %K RCC5585 %K RCC5586 %K RCC5587 %K RCC5588 %K RCC5589 %K RCC5590 %K RCC5591 %K RCC5592 %K RCC5593 %K RCC5594 %K RCC5595 %K RCC5596 %K RCC5597 %K RCC5598 %K RCC5599 %K RCC5600 %K RCC5601 %K RCC5602 %K RCC5603 %K RCC5604 %K RCC5605 %K RCC5606 %K RCC5607 %K RCC5608 %K RCC5609 %K RCC5610 %K RCC5611 %K RCC5612 %X Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single cell pipetting and serial dilution. Although all three techniques yielded the most common organisms, each technique retrieved specific taxa, highlighting the importance of using several methods to maximize the number and diversity of isolated strains. More than 1,000 cultures were obtained, characterized by 18S rRNA sequencing and optical microscopy and de-replicated to a subset of 276 strains presented in this work. Strains grouped into 57 genotypes defined by 100% 18S rRNA sequence similarity. These genotypes spread across five divisions: Heterokontophyta, Chlorophyta, Cryptophyta, Haptophyta and Dinophyta. Diatoms were the most abundant group (193 strains), mostly represented by the genera Chaetoceros and Attheya. The genera Rhodomonas and Pyramimonas were the most abundant non-diatom nanoplankton strains, while Micromonas polaris dominated the picoplankton. Diversity at the class level was higher during the peak of the bloom. Potentially new species were isolated, in particular within the genera Navicula, Nitzschia, Coscinodiscus, Thalassiosira, Pyramimonas, Mantoniella and Isochrysis. %B Elementa: Science of the Anthropocene %V 8 %P 6 %8 feb %G eng %U https://www.biorxiv.org/content/10.1101/642264v1 https://www.elementascience.org/article/10.1525/elementa.401/ %R 10.1525/elementa.401 %0 Journal Article %J Genome Biology and Evolution %D 2019 %T Tracing the evolution of the plastome and mitogenome in the chloropicophyceae uncovered convergent tRNA gene losses and a variant plastid genetic code %A Turmel, Monique %A dos Santos, Adriana Lopes %A Otis, Christian %A Sergerie, Roxanne %A Lemieux, Claude %E Archibald, John %K RCC15 %K RCC1871 %K RCC2335 %K RCC2339 %K RCC287 %K RCC3374 %K RCC3402 %K RCC4434 %K RCC4572 %K RCC4656 %K RCC696 %K RCC856 %K RCC998 %K RCC999 %B Genome Biology and Evolution %V in press %8 apr %G eng %U https://www.biorxiv.org/content/10.1101/530998v1 https://academic.oup.com/gbe/advance-article/doi/10.1093/gbe/evz074/5425330 %R 10.1093/gbe/evz074 %0 Journal Article %J Molecular Ecology Resources %D 2015 %T PhytoREF: a reference database of the plastidial 16S rRNA gene of photosynthetic eukaryotes with curated taxonomy %A Decelle, Johan %A Romac, Sarah %A Stern, Rowena F. %A Bendif, El Mahdi %A Zingone, Adriana %A Audic, Stéphane %A Guiry, Michael D. %A Guillou, Laure %A Tessier, Désiré %A Le Gall, Florence %A Gourvil, Priscillia %A dos Santos, Adriana Lopes %A Probert, Ian %A Vaulot, Daniel %A de Vargas, Colomban %A Christen, Richard %K 2015 %K MACUMBA %K rcc %K RCC?o?dd %K SBR$_\textrmP$hyto$_\textrmD$IPO %K SBR$_\textrmP$hyto$_\textrmE$PPO %K sbr?hyto$_\textrmd$ipo %K sbr?hyto?ppo %X Photosynthetic eukaryotes have a critical role as the main producers in most ecosystems of the biosphere. The ongo- ing environmental metabarcoding revolution opens the perspective for holistic ecosystems biological studies of these organisms, in particular the unicellular microalgae that often lack distinctive morphological characters and have complex life cycles. To interpret environmental sequences, metabarcoding necessarily relies on taxonomically curated databases containing reference sequences of the targeted gene (or barcode) from identified organisms. To date, no such reference framework exists for photosynthetic eukaryotes. In this study, we built the PhytoREF data- base that contains 6490 plastidial 16S rDNA reference sequences that originate from a large diversity of eukaryotes representing all known major photosynthetic lineages. We compiled 3333 amplicon sequences available from public databases and 879 sequences extracted from plastidial genomes, and generated 411 novel sequences from cultured marine microalgal strains belonging to different eukaryotic lineages. A total of 1867 environmental Sanger 16S rDNA sequences were also included in the database. Stringent quality filtering and a phylogeny-based taxonomic classifica- tion were applied for each 16S rDNA sequence. The database mainly focuses on marine microalgae, but sequences from land plants (representing half of the PhytoREF sequences) and freshwater taxa were also included to broaden the applicability of PhytoREF to different aquatic and terrestrial habitats. PhytoREF, accessible via a web interface (http://phytoref.fr), is a new resource in molecular ecology to foster the discovery, assessment and monitoring of the diversity of photosynthetic eukaryotes using high-throughput sequencing. %B Molecular Ecology Resources %V 15 %P 1435–1445 %G eng %U http://doi.wiley.com/10.1111/1755-0998.12401 %R 10.1111/1755-0998.12401