Carbon Isotope Fractionation in Noelaerhabdaceae Algae in Culture and a Critical Evaluation of the Alkenone Paleobarometer

TitleCarbon Isotope Fractionation in Noelaerhabdaceae Algae in Culture and a Critical Evaluation of the Alkenone Paleobarometer
Publication TypeJournal Article
Year of Publication2021
AuthorsPhelps SR, Hennon GMM, Dyhrman ST, Limón MDHernán, Williamson OM, Polissar PJ
JournalGeochemistry, Geophysics, Geosystems
Volume22
Paginatione2021GC009657
ISSN1525-2027
Keywordsalkenone, carbon dioxide, carbon isotope, coccolithophore, irradiance, paleobarometry, rcc, RCC1303
Abstract

The carbon isotope fractionation in algal organic matter ($\varepsilon$p), including the long-chain alkenones produced by the coccolithophorid family Noelaerhabdaceae, is used to reconstruct past atmospheric CO2 levels. The conventional proxy linearly relates $\varepsilon$p to changes in cellular carbon demand relative to diffusive CO2 supply, with larger $\varepsilon$p values occurring at lower carbon demand relative to supply (i.e., abundant CO2). However, the response of Gephyrocapsa oceanica, one of the dominant alkenone producers of the last few million years, has not been studied closely. Here, we subject G. oceanica to various CO2 levels by increasing pCO2 in the culture headspace, as opposed to increasing dissolved inorganic carbon (DIC) and alkalinity concentrations at constant pH. We note no substantial change in physiology, but observe an increase in $\varepsilon$p as carbon demand relative to supply decreases, consistent with DIC manipulations. We compile existing Noelaerhabdaceae $\varepsilon$p data and show that the diffusive model poorly describes the data. A meta-analysis of individual treatments (unique combinations of lab, strain, and light conditions) shows that the slope of the $\varepsilon$p response depends on the light conditions and range of carbon demand relative to CO2 supply in the treatment, which is incompatible with the diffusive model. We model $\varepsilon$p as a multilinear function of key physiological and environmental variables and find that both photoperiod duration and light intensity are critical parameters, in addition to CO2 and cell size. While alkenone carbon isotope ratios indeed record CO2 information, irradiance and other factors are also necessary to properly describe alkenone $\varepsilon$p.

URLhttps://onlinelibrary.wiley.com/doi/abs/10.1029/2021GC009657
DOI10.1029/2021GC009657