@article {chen_genetic_2013,
	title = {Genetic transformation of marine cyanobacterium Synechococcus sp. CC9311 (Cyanophyceae) by electroporation},
	journal = {Chinese Journal of Oceanology and Limnology},
	volume = {31},
	number = {2},
	year = {2013},
	pages = {416{\textendash}420},
	abstract = {Synechococcus sp. CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation (CA). A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA. The results show that Synechococcus sp. CC9311 cells were sensitive to four commonly used antibiotics: ampicillin, kanamycin, spectinomycin, and chloramphenicol. An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV, using a kanamycin resistance gene as selectable marker, was constructed by recombinant polymerase chain reaction. The plasmid was then transformed into Synechococcus sp. CC9311 via electroporation. High transformation efficiency was achieved at a field strength of 2 kV/cm. DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin. In addition, the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.},
	keywords = {RCC1086},
	issn = {1993-5005},
	doi = {10.1007/s00343-013-2164-5},
	url = {https://doi.org/10.1007/s00343-013-2164-5},
	author = {Chen, Huaxin and Lin, Hanzhi and Jiang, Peng and Li, Fuchao and Qin, Song}
}
