@article {moreno-cabezuelo_integrated_2023,
	title = {Integrated Proteomic and Metabolomic Analyses Show Differential Effects of Glucose Availability in Marine Synechococcus and Prochlorococcus},
	journal = {Microbiology Spectrum},
	year = {2023},
	month = {feb},
	pages = {e03275{\textendash}22},
	abstract = {<p>We compared changes induced by the addition of 100 nM and 5 mM glucose on the proteome and metabolome complements in Synechococcus sp. strains WH8102, WH7803, and BL107 and Prochlorococcus sp. strains MED4, SS120, and MIT9313, grown either under standard light conditions or in darkness. Our results suggested that glucose is metabolized by these cyanobacteria, using primarily the oxidative pentoses and Calvin pathways, while no proof was found for the involvement of the EntnerDoudoroff pathway in this process. We observed differences in the effects of glucose availability, both between genera and between Prochlorococcus MED4 and SS120 strains, which might be related to their specific adaptations to the environment. We found evidence for fermentation in Prochlorococcus sp. strain SS120 and Synechococcus sp. strain WH8102 after 5 mM glucose addition. Our results additionally suggested that marine cyanobacteria can detect nanomolar glucose concentrations in the environment and that glucose might be used to sustain metabolism under darkness. Furthermore, the KaiB and KaiC proteins were also affected in Synechococcus sp. WH8102, pointing to a direct link between glucose assimilation and circadian rhythms in marine cyanobacteria. In conclusion, our study provides a wide overview on the metabolic effects induced by glucose availability in representative strains of the diverse marine picocyanobacteria, providing further evidence for the importance of mixotrophy in marine picocyanobacteria.</p>
},
	keywords = {RCC156, RCC407, RCC515, rcc539, rcc752},
	issn = {2165-0497},
	doi = {10.1128/spectrum.03275-22},
	url = {https://journals.asm.org/doi/10.1128/spectrum.03275-22},
	author = {Moreno-Cabezuelo, Jos{\'e} {\'A}ngel and G{\'o}mez-Baena, Guadalupe and D{\'\i}ez, Jes{\'u}s and Garc{\'\i}a-Fern{\'a}ndez, Jos{\'e} Manuel},
	editor = {Hom, Erik F. Y.}
}
@article {Dominguez-Martin2017,
	title = {Quantitative proteomics shows extensive remodeling induced by nitrogen limitation in prochlorococcus marinus SS120},
	journal = {mSystems},
	volume = {2},
	number = {3},
	year = {2017},
	note = {tex.mendeley-tags: RCC156},
	month = {jun},
	pages = {e00008{\textendash}17},
	abstract = {Prochlorococcus requires the capability to accommodate to environmental changes in order to proliferate in oligotrophic oceans, in particular regarding nitrogen availability. A precise knowledge of the composition and changes in the proteome can yield fundamental insights into such a response. Here we report a detailed proteome analysis of the important model cyanobacterium Prochlorococcus marinus SS120 after treatment with azaserine, an inhibitor of ferredoxin-dependent glutamate synthase (GOGAT), to simulate extreme nitrogen starvation. In total, 1,072 proteins, corresponding to 57\% of the theoretical proteome, were identified{\textemdash}the maximum proteome coverage obtained for any Prochlorococcus strain thus far. Spectral intensity, calibrated quantification by the Hi3 method, was obtained for 1,007 proteins. Statistically significant changes ( P value of {\textexclamdown}0.05) were observed for 408 proteins, with the majority of proteins (92.4\%) downregulated after 8 h of treatment. There was a strong decrease in ribosomal proteins upon azaserine addition, while many transporters were increased. The regulatory proteins P II and PipX were decreased, and the global nitrogen regulator NtcA was upregulated. Furthermore, our data for Prochlorococcus indicate that NtcA also participates in the regulation of photosynthesis. Prochlorococcus responds to the lack of nitrogen by slowing down translation, while inducing photosynthetic cyclic electron flow and biosynthesis of proteins involved in nitrogen uptake and assimilation.},
	keywords = {RCC156},
	issn = {2379-5077},
	doi = {10.1128/mSystems.00008-17},
	url = {http://msystems.asm.org/lookup/doi/10.1128/mSystems.00008-17},
	author = {Dom{\'\i}nguez-Mart{\'\i}n, Maria Agustina and G{\'o}mez-Baena, Guadalupe and D{\'\i}ez, Jes{\'u}s and L{\'o}pez-Grueso, Maria Jos{\'e} and Beynon, Robert J. and Garc{\'\i}a-Fern{\'a}ndez, Jos{\'e} Manuel},
	editor = {Huber, Julie A.}
}
